Fig. 7: Human sepsis survivors exhibit reduced MICU1:MCU expression in immune cells. | Communications Biology

Fig. 7: Human sepsis survivors exhibit reduced MICU1:MCU expression in immune cells.

From: Sepsis restructures the mitochondrial calcium uniporter complex in the lymphoid tissues of mice and humans

Fig. 7

A Bacterial sepsis. Samples of blood were obtained from participants at day 30 after ICU admission and a primary diagnosis of either trauma (n = 7) or sepsis (n = 4). Peripheral blood mononuclear cells (PBMC) were isolated and analyzed by immunoblot for MICU1, MCU, and TOM20. Densitometry was performed using NIH ImageJ. bar = median. B SARS-CoV-2. Samples of blood were obtained from participants at day 7, 28 and 84 after a first sample (Day 0) collected early after ICU admission. Peripheral blood mononuclear cells (PBMC) were isolated and analyzed by immunoblot for MICU1, MCU, and TOM20. C PMBC [Ca2+]m in SARS-CoV-2. PBMC were isolated, analyzed by flow cytometry for immune subtypes and for [Ca2+]m using Rhod-2 (ex/em 552 nm/581 nm) Wilcoxon rank sum.

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