Fig. 4: NGS analysis identified new antiretroviral drug-resistant mutations. | Communications Biology

Fig. 4: NGS analysis identified new antiretroviral drug-resistant mutations.

From: Unique molecular signatures in rebound viruses from antiretroviral drug and CRISPR-treated HIV-1-infected humanized mice

Fig. 4

A Next-generation sequencing (NGS) was used to screen the low-frequency HIV mutants (less than 15% detection threshold) in the protease (PR), reverse transcriptase (RT), and integrase (IN) regions within pol amplicons. A total of 25 humanized mouse samples belonging to either HIV-1NL4-3 or HIV-1ADA-infected differential treatment groups from pre-ART and endpoint were subjected to the NGS analysis. B A heap map shows the predicted major antiretroviral drug susceptibility profiles based on the Stanford drug resistance database from HIV-1NL4-3 and HIV-1ADA-infected and differentially treated samples. (The colors used to indicate the results are red, high-level resistance; orange, medium-level resistance; pink, low-level resistance; blue, sensitive; dark purple, unclear functionality.) The four ARVs used for this study were highlighted with a red arrow.

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