Fig. 6: Next-generation sequencing identified new mutations in treated rebound animals.
A, B Distribution of new and unique mutations in the protease (PR), reverse transcriptase (RT), and integrase (IN) regions of rebound viruses belonging to the different treatment groups infected with either HIV-1 strains NL4_3 or ADA. Violin plots depict the frequency of new mutations in each region relative to the total mutations identified in the individual sample. The frequency of new mutations identified in PR, RT, and IN regions from (n = 16) samples (HIV-1NL4_3 group) and (n = 4) samples (HIV-1ADA group) was analyzed using Type III Analysis of Variance Table with Satterthwaite’s method (p values shown in the figure). Comparison between the two regions was performed using Student’s t-test (two-tailed, paired) (C–E). The pattern of polymorphism and mutations associated with new mutations identified in the RT region of HIV-1NL4_3 treatment groups. Bar plots represent the frequency of new mutations in the RT region across different treatment groups: LA ART, CRISPR, and the dual-treated rebound group. Each bar represents the frequency of a specific amino acid mutation at a consensus position, with the height of the bar corresponding to the percentage frequency, and those shared between groups are indicated by consistent color coding. The alphabets represent single-letter amino acid codes.
