Fig. 4: Application of the SPIDER-seq method to the library prepared using adaptor ligation. | Communications Biology

Fig. 4: Application of the SPIDER-seq method to the library prepared using adaptor ligation.

From: Correcting errors in PCR-derived libraries for rare allele detection by reconstructing parental and daughter strand information

Fig. 4

a Schematic illustration of CID-based barcoding for preparation of the shot-gun sequencing library. b Comparison of VAFs observed using SPIDER-seq with known VAFs provided by the manufacturer. For each sample and variant, the mean observed VAF from replicate experiments is presented. The line is a linear fit. Pearson r = 0.93944. c Identification of mutations from hybridization capture data at AF values of 1, 0.5, 0.25, and 0.125%. Each column corresponds to a single sample in a single replicate experiment.

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