Fig. 4: Apoptotic cells have enhanced procoagulant activity.
From: Apoptotic cells promote circulating tumor cell survival and metastasis
Time to fibrin clot formation was measured as the maximum change in absorbance (Time to Vmax) after adding Ca2+ to citrated mouse plasma containing live or apoptotic cells; lower values indicate more rapid clot formation (A, B, D, E). Met-1 or 3T3 expressing acCasp9 were left untreated or treated with B/B for 2 h (A, B, D–G). TF-PE fluorescent intensity was measured by flow cytometry (C). TF activity was measured as the absorbance at 30 min after addition of live or apoptotic cells to mouse FVII, human FX, and chromogenic FXa substrate (F, G). Circles connected by lines are matched serum samples, and statistical analysis is two-way ANOVA with Tukey’s multiple comparison test (B, D, E). Dotted line represents the absorbance of a control well containing all assay components without cells added; statistical analysis is Welch’s ANOVA with Dunnett’s multiple comparisons test, and error bars represent SEM (F, G). Diagram created in BioRender. Oberst, A. (2025) https://BioRender.com/fa409ue (A).
