Fig. 6: Tissue factor is responsible for the magnitude of apoptotic cell-enhanced metastasis and platelet clotting in the lung.

TF-PE fluorescent intensity was measured by flow cytometry on live cells (A, C). Surface lung metastasis was quantified at 14 days post I.V. injection (B, D). Lungs were harvested for fluorescent microscopy 1 h after I.V. injection, Cells were mixed up to 1 h in advance and injected in a single bolus (mixed), or injected in two sequential injections into opposite tail veins (separate), acCasp9 Met-1 cells were stained with CFSE (green), Met-1 cells expressed mCherry (red), platelets are stained with αGP1bβ (white), scale bar = 200 µm (E–I) Lines connect values from individual lung sections (F). Striped portion of the bar is the % of tumor cells in a clot that also contains an apoptotic cell (I). Dots are biological replicates (B, D) or multiple slices of lung from n = 3–4 biological replicates (F–I). Statistical testing is Ordinary one-way ANOVA with Tukey’s multiple comparisons test (B, D, F–H) or Welch’s ANOVA with Dunnett’s multiple comparisons test (I). Error bars represent SEM.