Fig. 1: Characterization of hMG cells.

a Representative ICC images of iPSC-derived hMG after 7 days in culture from CTL (SP09), L2-PD (L2-PD1: SP12) and L2-PD^corr (L2-PD1^corr: SP12wt/wt) iPSC lines staining positive for IBA-1, CX3CR1 or TMEM-119 (green) and negative for astrocytic (GFAP) or neuronal (TUJ1) markers. Nuclei are counterstained with DAPI (blue). Scale bar = 30 μm. b Percentage of hMG cells from CTL (SP09), L2-PD (L2-PD1: SP12; L2-PD2: SP13) and L2-PD^corr (L2-PD2^corr: SP13wt/wt) lines expressing IBA-1, CX3CR1 or TMEM-119 in respect to DAPI. Individual data plotted, along with mean ± SEM. N = 3 of independent experiments, each experiment containing two technical duplicates. c Schematic representation of LPS stimulation (100 ng/ml). Cytokine profile for IL-1β (d), IL-6 (e) and TNF-α (f) of hMG from CTL (SP09), L2-PD (L2-PD1: SP12; L2-PD2: SP13) and L2-PD^corr (L2-PD2^corr: SP13wt/wt) iPSC lines after 24 hours of LPS stimulation. Individual data plotted, along with mean ± SEM. One-way ANOVA with Uncorrected Fisher LSD test. N = 3 of independent experiments, each experiment containing two technical duplicates. g Representative Bright Field and Red fluorescent images of CTL (SP09), L2-PD1 (SP12), L2-PD1^corr (SP12wt/wt), L2-PD2 (SP13), and L2-PD2^corr (SP13wt/wt) hMG phagocyting pHrodo labelled SYNs after 150 minutes of exposure to SYNs (Scale bar = 25 μm). h pHRodo fluorescence intensity within hMG after 300 minutes comparing CTL (SP09), L2-PD1 (SP12), L2-PD1^corr (SP12wt/wt), L2-PD2 (SP13), and L2-PD2^corr (SP13wt/wt) hMG. Individual data plotted, along with mean ± SEM. Kruskal-Wallis non-parametric test with Uncorrected Dunn’s test. N = 3 of independent experiments, for some experiments two technical duplicates were measured. *p < 0.05, **p < 0.01. p-values over 0.1 (non-significant) are not shown.