Fig. 4: Microglial activation is increased in L2-PD patient post-mortem brains. | Communications Biology

Fig. 4: Microglial activation is increased in L2-PD patient post-mortem brains.

From: LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSC-based Parkinson’s disease model

Fig. 4

a Representative image of IBA-1+ cells (in blue; unstained NM in brown) from control, iPD and L2-PD human brains showing ramified (white arrowhead) or amoeboid (black arrowhead) morphologies. Grey arrowheads indicate NM-laden neurons. Scale bar = 50 μm; 20 μm. b Total number of IBA-1+ cells/mm2 in post-mortem human brains from control subjects, iPD and L2-PD patients. c Number of IBA-1+ cells/mm2 displaying amoeboid morphologies in control, iPD and L2-PD brains. d Number of IBA-1+ cells/mm2 displaying ramified morphologies in control, iPD and L2-PD brains. e Difference in the number of IBA-1+ cells/mm2 displaying amoeboid morphologies in areas close to rNM vs areas distant from it. f Representative image of a quantification area close to rNM. N = 30 Control subjects, N = 9 iPD patients and N = 6 L2-PD patients. Individual data plotted, along with mean ± SEM. Individual data plotted, along with mean ± SEM. Scale bar = 25 μm. In b and c, One-Way ANOVA with Tukey’s multiple comparison test; In e, Kruskal-Wallis with Dunn’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001. p-values over 0.1 (non-significant) are not shown.

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