Fig. 2: Analytic rules of STRaM.

A Inclusivity of SNPs in the repeat marginal regions ensures the continuation of repetitive sequences, e.g. the DS5818 and D13S317 loci, n = 16 cell line alleles. B STRaM rules encompass CE rules via an annexation of the repeat units with SNPs. C, D A schematic illustration of independent STR analysis and the STR flanking analysis. Both analyses occasionally run into errors, C a misidentification of VWA in the A549 cells and (D) failure to align merged reads of the T47D cells to the 3' reference flanking sequence of D2S437. E Error checks of STR analysis and STR flanking analysis via DSP, CSL and FRC as evaluated, respectively, from the genomic coordinates, STR length and read counts. F Nucmer outputs filtered out by the different sizes of aligned sequences. G Accuracy comparison of the output results of STR-FM, Flanking alignment and STRaM for true sequences using the ASN-0002 STR panel and STRaM panel loci, Error bar, mean ± SEM.