Fig. 7: STRaM for monitoring and tracking in cell-based research and therapy. | Communications Biology

Fig. 7: STRaM for monitoring and tracking in cell-based research and therapy.

From: STRaM: A genetic framework for improved cell product provenance for research and clinical translations

Fig. 7

A STRaM analysis applied to cell line analysis, preclinical model analysis and cell therapy monitoring. B Comparison of preclinical PDO and PDX cells to parental tumors (n = 3 patients). C In vitro expansion of MSC was evaluated by STRaM analysis (n = 3 volunteers). D Scheme for autologous CAR-T cell therapy. E-G CAR-T cells were monitored via STRaM analysis for (E) authentication (SI = 100 ± 0.0%, all samples), (F) purity test (PI = 97.7 ± 0.9%, all samples), and (G) relative levels of CAR genes were increased after CAR-T infusion (n = 3 patients). The PBMCs 5 day before infusion (-5) were used as parental controls for SI and PI. The data were analyzed by student’s t-test. PBMCs, peripheral blood mononuclear cells. Error bars, mean ± SEM.

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