Fig. 8: CSF-cNs express differential GABA_A and glycine receptors density along the central canal axis.

A Representative whole-cell current traces recorded at V_h -80 mV in response to pressure application of GABA (1 mM, 30 ms, arrow) in control (black traces) and in the presence of gabazine (+ Gbz, 10 µM; violet traces), a selective GABA_A receptor antagonist, in CSF-cNs from the C, T and L segments (Left to Right). B Summary boxplots with whiskers of the average amplitude of the GABA-mediated current densities recorded in control at V_h -80 mV (C: N = 2, n = 14; T: N = 2, n = 10 and L: N = 2, n = 12). Kruskal-Wallis rank sum test: χ² = 17.975, df = 2, p(χ²) = 1.25 × 10⁴ and post hoc pairwise comparisons using Wilcoxon rank sum test with continuity correction: C vs. T, p = 0.02602; C vs. L, p = 0.00306 and T vs. L, p = 0.00011). C Representative whole-cell current traces recorded at V_h -80 mV in response to pressure application of glycine (1 mM, 30 ms, arrow) in control (black traces) and in the presence of strychnine ( + Stry,10 µM; violet traces), a selective glycine receptor antagonist, in CSF-cNs from the C, T and L segments (Left to Right). D Summary boxplots of the averaged amplitude of the glycine-mediated current densities recorded in control at V_h -80 mV (C: N = 2, n = 11; T: N = 9, n = 10 and L: N = 6, n = 12). Kruskal-Wallis rank sum test: χ² = 14.979, df = 2, p(χ²) = 5.589 × 10⁴ and post hoc pairwise comparisons using Wilcoxon rank sum test with continuity correction: C vs. T, p = 0.03; C vs. L, p = 5.3 × 10⁻⁵ and T vs. L, p = 0.25). In B and D, Single data points for all the recorded cells at each level are presented with colored opened circle (same color code as above).