Fig. 9: CSF-cNs differentially express slow kinetics-low amplitude AMPA/kainate glutamatergic receptors that modulate their excitability. | Communications Biology

Fig. 9: CSF-cNs differentially express slow kinetics-low amplitude AMPA/kainate glutamatergic receptors that modulate their excitability.

From: Cerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cord

Fig. 9

A Representative whole-cell current traces recorded in CSF-cNs from the C, T and L segments (Top to Bottom) at Vh -80 mV in response to pressure application of glutamate (100 µM, 100 ms and 500 ms for L; A1), AMPA (100 µM, 30 ms; A2) and kainate (100 µM, 30 ms; A3). In A1, the representative currents recorded in the presence of DNQX (100-400 µM; violet traces) upon glutamate applications are illustrated for each segment below the control traces. Arrows and dashed lines in A1, A2 and A3 indicate time of agonist application. B Summary boxplots of the average amplitude of the glutamate-mediated currents recorded at Vh -80 mV in control (Left) and in the presence of DNQX (Right) for the 3 segments of interest (C: N = 2, n = 11; T: N = 2, n = 5 and L: N = 2, n = 7). ANOVA.lme: F = 13.83, df=5 and 40, p(F) = 7.362 × 10−6 and Tukey (EMM) post hoc test to compare glutamate-mediated currents densities between regions (C vs. T, p = 0.8119; C vs. L, p = 0.005 and T vs. L, p = 0.0008; black asterisk and bars) and the current densities recorded in control and in the presence of DNQX (CTR vs. DNQX, p = 2.892 × 10−8; violet bars and asterisks). C Summary boxplots of the averaged amplitude of the current densities recorded at Vh -80 in CSF-cNs from the C, T and L segments in response to pressure application of glutamate (Glu), AMPA and kainate (C: N = 3, n = 16, 11 and 11; T: N = 3, n = 13, 4 and 5 and L: N = 3, n = 17, 7 and 6; Data and n are given for Glu-, AMPA- and -kainate-mediated current, respectively). ANOVA.lme: F = 7.907, df=8 and 81, p(F) = 8.36 × 108 and Tukey (EMM) post hoc test to compare Glu-, AMPA- and kainate-mediated currents between the different regions; Glu: C vs. T, p = 0.0938; C vs. L, p = 0.0170 and T vs. L, p < 0.0001; AMPA: C vs. T, p = 0.074; C vs. L, p < 0.0001 and T vs. L, p = 0.2032; kainate: C vs. T, p = 0.8941; C vs. L, p = 0.1516 and T vs. L, p = 0.4731). D Representative traces of the membrane potential changes recorded at Vm -60 mV in current-clamp mode in CSF-cNs from the C, T and L segments (Left to Right) in response to pressure application of glutamate (100 µM, 100 ms). Bottom traces in violet are membrane potential changes recorded upon glutamate application in the presence of DNQX. Shaded colored bars indicate the duration of the agonist application and dashed lines the 0 mV voltage. E1 Summary boxplots of the average depolarization levels of the membrane potential in from the C- (N = 2, n = 5), T- (N = 3, n = 7) and L-CSF-cNs (N = 2, n = 9) in response to pressure application of glutamate. Kruskal-Wallis rank sum test: χ2 = 11.957, d = 2, p(χ2) = 2.533 × 103 and post hoc pairwise comparisons using Wilcoxon rank sum test with continuity correction: C vs. L, p = 0.530; C vs. T and T vs. L, p = 0.005). E2 Summary box-and-whiskers plots of the average number of AP triggered in CSF-cNs from C- (N = 2, n = 5), T- (N = 3, n = 7) and L-CSF-cNs (N = 2, n = 9) in response to pressure application of glutamate. Kruskal-Wallis rank sum test: χ2 = 7.5111, df=2, p(χ2) = 0.02339 and post hoc pairwise comparisons using Wilcoxon rank sum test with continuity correction: C vs. T, p = 0.302; C vs. L, p = 0.034 and T vs. L, p = 0.117). In B, D and E. Single data points for all the recorded cells at each level are presented with colored opened circle (same color code as above).

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