Fig. 5: RPS29 serves as a quality control regulator of protein translation. | Communications Biology

Fig. 5: RPS29 serves as a quality control regulator of protein translation.

From: Machine learning-based proteomics profiling of ALS identifies downregulation of RPS29 that maintains protein homeostasis and STMN2 level

Fig. 5

Immunoblots a and quantification results b showing the translation rate detected by puromycin incorporation assay in SH-SY5Y cells after RPS29 knockdown. Each dot represents one biological replicate (n = 3). Data are presented as mean ± SD. Immunoblots c and quantification results d showing the protein ubiquitination levels detected by ubiquitin antibody in SH-SY5Y cells after RPS29 knockdown. Each dot represents one biological replicate (n = 3). Data are presented as mean ± SD. e Relative neuronal viability of differentiated SH-SY5Y with shRNA-mediated knockdown of RPS29. The differentiation protocol was also showed on the top. Each dot represents one biological replicate (n = 3). Data are presented as mean ± SD. f A scheme of the dual luciferase reporter and control reporter to measure stop codon readthrough and misincorporation translational errors. g Translation accuracy levels measured in SH-SY5Y cells with shRNA-mediated knockdown of RPS29. Top, stop codon readthrough assay. Bottom, misincorporation translational error assay. Each dot represents one biological replicate (n = 6). All data are representative of at least three independent experiments. Data are presented as mean ± SD. The p value was calculated using a two-tailed t-test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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