Fig. 5: Adjuvant activity of NSF-951 in vivo.
From: Adjuvant activity of a small molecule TLR4 agonist discovered via structure-based virtual screening
Antigen specific immune response in mice immunized with OVA formulated with compounds. Mice were immunized with OVA formulated in NSF-951 or NSF-418, or NSF-501 or MPLA, and antigen-specific Antibody response (A) in the serum obtained at day 28 was determined by ELISA and cellular immune response was determined by analysing the levels of IL-4 (B) and IFN-γ (C) in the culture supernatant of the stimulated spleenocytes using were analysed by ELISA as detailed in the materials and methods section and was determined as described in materials and methods. (Antigen-specific immune response in mice immunized with OVA formulated with AF007. D Pictorial combination of ASO4 (Alum +MPLA) and AF007 (Alum +NSF951). Mice were immunized with OVA formulated with Alum and NSF-951 (AF007) or MPLA (AS04), and antigen-specific Antibody response (E), Isotypes (F) in the serum obtained at day 28 was determined by ELISA and cellular immune response was determined by analysing the levels of IL-4 (G) and IFN-γ (H) in the culture supernatant. All experiments were performed in two independent biological replicates with four animals per group (n = 4). Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA followed by Dunnett’s post hoc test. Significance is indicated as follows: ***P < 0.001, ****P < 0.0001, and ns (not significant).
