Fig. 2: Identification of candidate genes underlying iWUEFL through GWAS. | Communications Biology

Fig. 2: Identification of candidate genes underlying iWUEFL through GWAS.

From: The phytochrome-interacting factor PIL13 enhances water use efficiency under fluctuating light and drought resilience in rice and soybean

Fig. 2

Zoom-in Manhattan plots of iWUEFL for field (A) and GC (B) conditions. The SNPs in the OsPIL13 gene above the threshold of significant association were highlighted in red scatters. C List of six candidate genes that are located within the linkage disequilibrium (LD) block and their genomic positions. D Response of candidate genes, within the LD block surrounding the lead SNP, to FL-DS in two groups possessing contrasting iWUEFL values. Plants were exposed to HL for 30 min, followed by 20 min LL, for qPCR experiments. Each group has 10 rice accessions in the Minicore population. Data are mean ± s.d. (n = 3 independent replicates). Student’s t-test was used to determine the significance level of the LL response of each gene between the two iWUEFL subgroups. E OsPIL13 gene expression levels followed by HL to LL transition treatment within 10 min between A4003 and T4178. Data are mean ± s.d. (n = 3 independent replicates). F Protein expression of OsPIL13 following HL to LL transition within 10 min in leaves of WYG7. G Heatmap representing the relative gene expression of 13 orthologous genes of PIL13 in the rice genome in leaves of WYG7 exposed to either HL or LL conditions. The values for each cell are derived from three biological replicates.

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