Fig. 3: Haplotype analysis of OsPIL13.

A Structure and haplotype analysis of OsPIL13. Distribution of iWUE_FL in the field (B) and in GC (C) in two haplotypes of the OsPIL13 gene. The dotted lines represent the 0.25 and 0.75 quantiles, with median values indicated by bold lines. Differences between haplotypes were statistically analyzed using Student’s t test. The number of plants carrying Hap I and Hap II is shown in brackets. D Subpopulation distribution in two haplotypes of the OsPIL13 gene. E Response of OsPIL13 promoter activity to light during the switch from HL to LL within 10 min. Hap II and Hap I represent the two versions present in the OsPIL13 promoter. Protoplast was exposed to HL for 30 min, followed by 20 min of LL. F Stimulated expression of OsPIL13 by LL with different variations in the OsPIL13 promoter. Values were calculated using the ratio of luciferase activities at 5 min of LL exposure against 0 min LL for each SNP version. The symbol “v(x)ml” represents six mutation versions introduced from Hap II into Hap I of the OsPIL13 promoter. For panels (E, F), data are mean ± s.d. (n = 3 independent replicates). For panel (E), the symbols “*”, “**” and “***” were used to represent the significant differences for each time point at P < 0.05, P < 0.01, and P < 0.001, respectively, based on Student’s t test. For panel (F), different letters indicate significant differences based on one-way ANOVA followed by Tukey’s HSD tests (P < 0.05).