Fig. 3: Mitochondrial supercomplexes (SCs) of frozen left ventricular tissue homogenates of 6-month-old Nntwt mice using blue native gel electrophoresis (BNGE) followed by immunoblotting.
From: A robust method for assessing mitochondrial function in healthy and diseased frozen cardiac tissue
A Representative images of mitochondrial CI-CIV SCs (colored yellow) and individual bands corresponding to CI (colored green) and CIV (colored red). B No CII-CIV SCs were found due to technical limitations of BNGE. Individual bands corresponding to CII (colored green) and CIV (colored red) could be observed. C Representative images of SCs formed with CS and CI (colored yellow) and individual bands corresponding to CI (colored green) and CS (colored red). D Representative images of SCs formed with CII and CS (colored yellow). Individual bands corresponding to CII (colored green) and CS (colored red). Nntwt BL6JRcc(BL6J)-Nnt+/Wuhap mice, CI complex I, CII complex II, CIV complex IV, CS citrate synthase.
