Fig. 1: The integrity of the ARH is required for the enhancement of saccharin intake induced by calorie restriction.
From: AgRP neuron activity enhances reward-related consummatory behaviors during energy deficit in mice
a The experimental timeline summarizes the design used in the current study. Male mice were calorie restricted to 40% of their basal intake for 5 days and had restricted access to a saccharin solution (0.1%) for 4 h every day while water was ad libitum available. During the limited access period, chow was removed from home cages. Mice were perfused on day 5, prior to any access to saccharin or food. b–e Show food intake (b, p-time x group < 0.001, Cohen’s f = 1.984), body weight change, (c, p-time x group < 0.001, Cohen’s f = 3.084), saccharin intake (d, p-time x group < 0.001, Cohen’s f = 1.49) and saccharin preference (e, p-time x group < 0.001, Cohen’s f = 1.013), of wild-type (WT) mice that were maintained with ad libitum access to regular chow or calorie restricted (CR) for 5 days (n = 5 mice per group). f, g Show saccharin intake (f, p-time x treatment = 0.060, Cohen’s f = 0.341; p-time x group < 0.001, Cohen’s f = 0.724; p-treatment x group = 0.0146, Cohen’s f = 0.464; p-time x treatment x group = 0.070, Cohen’s f = 0.334) and cumulative saccharin intake (g, p-group x treatment = 0.015, Cohen’s f = 0.515) of WT ARH-intact or ARH-ablated mice that were maintained with ad libitum access to regular chow (n = 5 and 7 mice per group) or CR (n = 7 and 11 mice per group). Data represent the mean ± SEM, with error bars representing the SEM, and were compared by two-way ANOVA (b–e, g) or three-way ANOVA (f). *, p < 0.05 vs. same treatment different group; #, p < 0.05 different treatment same group.
