Fig. 3: Calorie restriction induces c-Fos expression in AgRP neuronal targets.
From: AgRP neuron activity enhances reward-related consummatory behaviors during energy deficit in mice
a, c Bar graph displaying the quantitative analysis of the number of c-Fos+ cells in the paraventricular hypothalamic nucleus (PVH; p = 0.011, Cohen’s d = 0.99) and bed nucleus of the stria terminalis (BNST; p < 0.001, Cohen’s d = 3.22), respectively, of wild-type (WT) mice ad libitum fed (n = 5 and 4 mice per group) or calorie restricted (CR) (n = 5 and 4 mice per group). b, d Representative photomicrographs of the coronal section of PVH and BNST, respectively, subjected to chromogenic immunohistochemistry against c-Fos (brown signal, blue head arrows). e, g Bar graph displaying the quantitative analysis of the fluorescent area in the PVH (p = 0.801) and BNST (p = 0.0013, Cohen’s d = 3.92), respectively, of WT mice ad libitum fed or CR (n = 4 and 6 mice per group). f, h Representative photomicrographs of the coronal section of PVH and BNST, respectively, of WT mice subjected to fluorescent immunohistochemistry against AgRP (red). Scale Bar: 50 µm low magnification and 25 µm high magnification. Data represent the mean ± SEM, with error bars representing the SEM. Data were compared by t-test. *, p < 0.05 vs. ad libitum fed. aca, anterior commissure, anterior part.
