Fig. 5: Metastatic gene expression is shaped by oncogenic driver and genetic background.

a Unsupervised PCA of RNA-seq data from PT and Lung met tissues across all FVB driver and PyMT-F1 mouse models. Unsupervised PCA of individual PT and lung met samples from FVB Her2 (b), FVB C3Tag (c), FVB Myc (d), and FVB PyMT (e). f Histogram of the number of alternatively expressed genes in lung mets vs. PT for each FVB driver model (FDR < 0.05). Volcano plots with significantly up regulated genes in green and down regulated genes in yellow for MSGE in FVB Her2 (g), FVB C3Tag (h), FVB Myc (i), and FVB PyMT (j). k Histogram showing the enrichment score for commonly enriched pathways from MSGE of the C3Tag, Her2, and PyMT models. l Histogram showing the fold change of genes common to pathways enriched by MSGE. Unsupervised PCA of individual PT and lung met samples from BL6 (m), MOLF (n), CAST (o), BALB (p), SEAGN (q), and BL10 (r). s Volcano plots with significantly up regulated genes in green and down regulated genes in yellow for MSGE in the PyMT-F1 models. t Histogram of the number of alternatively expressed genes in lung mets vs. PT for each PyMT-F1 model (FDR < 0.05). u Histogram showing the enrichment score for commonly enriched pathways from MSGE of the F1-PyMT models. Histograms showing the fold change of common MSGE enriched in the Focal Adhesion (v) and Renin Secretion pathways (w).