Fig. 3: The interaction of Fis1−Bap31 in vitro. | Communications Biology

Fig. 3: The interaction of Fis1−Bap31 in vitro.

From: Crystal structure of Fis1 and Bap31 provides information on protein-protein interactions at mitochondria-associated ER membranes

Fig. 3

A The immunoprecipitation assay was performed with overexpressed full-length construct of FLAG-Fis1 and 2 different constructs of HA-Bap31 including full-length (FL) and C-terminal region truncated (ΔC), in the cells using FLAG-Fis1 as bait. Each dot in the graph (right) represents an individual biological replicate (n = 4), p value = 0.0011 (**); error bars represent standard deviation (SD). B The interaction of different constructs of Fis1_ΔTM and Bap31_CC2 was validated with SPR experiments by using purified recombinant proteins (WT and mutants of Fis1_ΔTM and Bap31_CC2). The SPR data were processed using equilibrium binding analysis instead of kinetic analysis due to the weak protein-protein interactions. All SPR experiments were performed in triplicate (n=3), and data are presented as mean ± standard error (SE). n.d: not determined. All source data are available in the Supplementary Information.

Back to article page