Fig. 4: Ym1 regulates keratin production in lesional skin. | Communications Biology

Fig. 4: Ym1 regulates keratin production in lesional skin.

From: Macrophagic Ym1 orchestrates γδT cell-derived IL-17 production and keratinocyte functionality to mediate psoriasis-like skin inflammation

Fig. 4

RNA-seq analysis was performed using skin tissue of IMQ-treated BR.Ncf1* (n = 3, designated as B1, B2, and B3) and BR.Ncf1*.Ym1Δ (n = 4, designated as Congenic1, Congenic2, Congenic3 and Congenic4) mice. The volcano plot of identified genes (a) and the number of differentially expressed genes (b) were shown. c K-means clustering of samples from two groups with different genotypes. d Gene expression of identified chitinases and chitinase-like proteins was compared between two groups. ***p < 0.001. e GO enrichment of differentially expressed genes. f Differentially expressed keratins and keratin-associated proteins were compared by a VlinPlot tool. g Keratin filament pathways were enriched by using gene set enrichment analysis (GSEA). h Immune cells in skin were sketched by the analysis of Immune Cell Abundance Identifier (ImmuCellAI). i PPAR pathways were enriched by GSEA. Data were analyzed by using the online OmicStudio tools (LC Bio Technology CO.,Ltd).

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