Fig. 5: Ym1 regulates keratinocyte functionality.
Primary keratinocytes from BR.Ncf1*.Ym1Δ mice were stimulated by rYm1 proteins with different concentrations, or by M5 cytokine cocktails. The mRNA expression of genes related to inflammatory response, proliferation and differentiation were determined by qPCR after 24 h stimulation, shown in heatmap (a) and histograms (b–d). n = 4. e Proliferation-related Keratins in cells was also determined by Western blotting. n = 3. f Cell viability was measured by CCK-8 assay in KCs with 24 h and 48 h stimulation. n = 5. g Cell proliferation was determined by EdU assay in KCs with 48 h stimulation. n = 5. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
