Fig. 7: Theta-burst stimulation modulated short-term plasticity for Experiment A.
a Graphical summary of TBS protocol. i-The TBS was performed four times spaced by 13 minutes. ii-Within each TBS there are 10 trials with four spikes per trial. iii-The schematic of each trial. b Percent active area before and after stimulation across all 6 wells. Wells 4A–6A show consistent increase or decrease in active area in response to stimulation while wells 1A–3A show little change. c Representative heat map evoked activity response for wells 4A–6A. Bin size is equal to 10 ms. The stimulation pulses are the light grey vertical lines, and the dashed orange lines indicate the start/stop time of the analysis window for calculating evoked activity. d percentage of active electrodes, total spikes, and evoked activity for wells 1A-3A and then 4A-6A. Purple circle represents well 1A, pink square – well 2 A, turquoise triangle – well 3A, green circle – well 4A, blue square – well 5A, and yellow triangle – well 6A. The data represents the mean with 10th to 90th percentile for each well. The 90th percentile response of a well treated with NBQX/AP5 before and during stimulation is shown with a blue dashed line overlayed on all graphs. The mean response of a well-treated with NBQX/AP5 before and during stimulation is shown in a black dashed line overlayed on all graphs. The 10th percentile response of a well treated with NBQX/AP5 before and during stimulation is shown in a red dashed line overlayed on all graphs. Responses above this NBQX/AP5 region indicate responses generated by glutamatergic receptors. e Histograms of total evoked activity per bin (bin size of 10 ms), total spikes, and total active area. The top three graphs show data aggregated across all electrodes for all 4 TBS for wells 1A–3A, and the bottom three graphs show data aggregated across all electrodes for all 4 TBS for wells 4A-6A. Wells 1A-3A show little to no response while wells 4A-6A indicate evoked responses on the millisecond timescale.
