Fig. 2: Rational designed and cell-free expressed bacteriocin cocktail.
From: Multiplexed bacteriocin synthesis to combat and prevent antimicrobial resistance
A Bacteriocins pathways in E. coli cell envelope. Tol and Ton-dependent bacteriocin pathways are highlighted in orange and blue, respectively, as well as producer cells and bacteriocins targeting those pathways. Different colicins and microcins are distinguished with the Col and Mcc prefixes, respectively, while post-translationally modified bacteriocins are indicated with an asterisk (*). B Activity of different bacteriocins against E. coli BW25113. Halo of activity as seen by the naked eye (left) and under a stereo microscope to detail colonies appearing in the halos (right). C Growth (OD600) of E. coli BW25113 in liquid cultures supplemented with single and co-expressed bacteriocins. D Viability (CFU ml−1) of cells in cultures (from C) after 72 h. E CFU counts of E. coli BW25113 in liquid cultures supplemented with single and co-expressed bacteriocins 1250-fold diluted. Lines in (C and E) and bars in (D) represent the average of three biological replicates \(\pm\) SD. Dots in (D) show the values of replicates.
