Fig. 1: Deletion of S1P in skeletal muscle increases sarcolipin expression and does not impact baseline SR calcium flux.
From: Site-1 protease is a negative regulator of sarcolipin promoter activity
A Volcano plot of genes identified from RNA-Seq as significantly differentially increased (red dots) and decreased (blue dots) in gastrocnemius of S1PsmKO mice relative to WT mice. Sarcolipin is indicated. n = 4 per genotype. B qPCR of sarcolipin mRNA expression in gastrocnemius of S1PsmKO and WT mice, n = 5 per group. Western blots with corresponding densitometry of sarcolipin protein levels in (C) gastrocnemius and (D) quadriceps of S1PsmKO and WT mice. n = 3–5 per group. E Peak tension of twitch and tetanic contractions and time to fatigue of S1PsmKO and WT gastrocnemius. F Rates of ATP-stimulated free calcium SR uptake with area under the curve and uptake half-life at baseline. Graphs are normalized to cyclopiazonic acid (CPA)- treated samples. n = 4, 5 per group. Data are reported as ± SEM. Statistical significance was determined by an unpaired t-test with Welch’s correction for unequal variances and a Wilcoxon two-sample test.
