Fig. 3: S1P inhibits sarcolipin transcription by activating ATF6.
From: Site-1 protease is a negative regulator of sarcolipin promoter activity
A Western blot of cells transfected with EV or constitutively active ATF6 (nATF6-HA). Representative blot of n = 3. B Sarcolipin promoter activity in cells expressing EV or nATF6. n = 5 per group. C Sarcolipin promoter activity in scrambled + EV, S1P knockdown + EV, and S1P knockdown + nATF6 cells. n = 5 per group. D ATF6-dependent transcription reporter activity in cells expressing EV, nATF6, or ATF6S1Pmut. n = 5 per group. Sarcolipin promoter activity in E cells expressing EV, nATF6, or ATF6S1Pmut and F cells expressing EV, CnA, or nATF6 as indicated. n = 5 per group. EV empty vector, CnA constitutively active calcineurin, ATF6S1Pmut ATF6 with mutated S1P cleavage site. Data are reported as ± SEM. Statistical significance was determined by unpaired t-test and unpaired t-test with Welch’s correction for unequal variances where necessary.
