Fig. 3: Matrisome distribution is altered in developing forelimbs lacking muscle.

A, B’ EMILIN1⁺ and WGA⁺ fibers (arrowheads) were observed in the tendons of both WT and Pax3^Cre/Cre embryos at E13.75. C, D’ Forelimb tendons from WT, but not Pax3^Cre/Cre, forelimbs were POSTN⁺ (arrow). E, F”’ FBN2 was still present in the ScxGFP⁺ area in Pax3^Cre/Cre forelimbs, whereas THBS4 was not. Dotted line delineates region of triceps muscle. Magnification 25× (A–D), 10× (E, F), 3D rendering of decellularized limbs z = 110 µm (A–D), cryosections (E–F”’), scale bars = 100 µm. U ulna, Hu humerus. Representative images from N = 3 independent biological replicates.