Fig. 2: Increased aggression in T2Dexo-PDOs compared to NDexo-PDOs. | Communications Biology

Fig. 2: Increased aggression in T2Dexo-PDOs compared to NDexo-PDOs.

From: Plasma exosomes from individuals with type 2 diabetes drive breast cancer aggression in patient-derived organoids

Fig. 2

A Bubble plot summarizing statistically significant gene sets enriched in differentially expressed gene sets between T2Dexo-PDOs (blue) and NDexo-PDOs (orange) identified with GSEA. Size of dot is scaled to adjusted p-value. B GSEA enrichment plots for representative gene sets upregulated (top) and downregulated (bottom) in T2Dexo-PDOs compared to NDexo-PDOs. C Violin plots of COSMIC CGC (left) and canonical CSC gene expression (right). Significance calculated via linear mixed effects model, ***<0.001, **<0.01, *<0.05. D Violin plots of top differentially expressed genes. E Representative confocal images of UT (left), NDexo (middle), and T2Dexo (right) per patient. Scale bar = 100 μm. F Violin plots of calculated circularity of PDOs. Singlets were removed during thresholding. Significance calculated via pairwise Wilcoxon Rank Sums test with Bonferroni correction, ***<0.001, **<0.01, *<0.05. G Projection of composite gene signatures capturing diabetes-associated exosomal effects onto TCGA (left) and METABRIC (right) cohorts. Cox proportional hazards model adjusted for age, proliferation score, inflammation score, and molecular subtype.

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