Fig. 3: No evidence of phosphorus mineralization function upregulation in bacterial genes correlated with arbuscular mycorrhizal fungal (AMF) community.

a The heatmap showing relative abundance of AMF correlated bacterial genes involving in phosphorus cycling in each plot (n = 34). Note total of six genes showed an increase in relative abundance upon nitrogen addition. Among them, one gene (olpA) is involved in organic phosphoester hydrolysis, one (ADE2) in purine metabolism, one (pckG) in pyruvate metabolism, one (rtpR) in pyrimidine metabolism, and two (phnS and phnV) in transporters. In contrast, five genes exhibited a decrease in relative abundance with nitrogen addition, which function in organic phosphoester hydrolysis (phoN), phosphonate and phosphinate metabolism (phnH), purine metabolism (ppx and purD), and transport (glpT). b, c Number of acid phosphatase genes and enzyme activity of acid phosphatase along a nitrogen addition gradient (n = 34). b Nitrogen addition increased the KO number of olpA and decreased that of phoN, two acid phosphatase genes. Note the number of phoN gene was about tenfold higher than olpA gene. c The measured activity of acid phosphatase showed no significant increase with nitrogen addition. d A schematic graph showing the up and down regulations of AMF correlated bacterial genes involving in phosphorus cycling in response to nitrogen addition. This graph displayed the chemical process catalyzed by the enzyme of the 11-phosphorus cycling related bacterial genes significantly correlated with AMF community as shown in Fig. 3a. Note we found no evidence of phosphorus mineralization function upregulation in bacterial genes associated with AMF community.