Fig. 1: Study design, dataset characterization and cell type identification. | Communications Biology

Fig. 1: Study design, dataset characterization and cell type identification.

From: Single-cell profiling of H3K4me1-H3K27me3 revealed bivalent regulation of abnormal neuronal development caused by prenatal e-cigarette vaporing

Fig. 1

A Overview of study design. Details can be found in the Results and Methods sections. The diagrams of rat brain and Paired-Tag flow were created with BioRender.com. B UMAP visualizing 15 cell types clustered from 14,170 single nuclei of P7 rat brain PFC. OPCs oligodendrocyte progenitor cells, ECs endothelial cells, CRs Cajal-Retzius cells, IT intratelencephalic excitatory neurons, PT pyramidal tract excitatory neurons, NP near projecting excitatory neurons, CT corticothalamic excitatory neurons. C Dot plot showing the expression of representative marker genes across all 15 cell clusters derived from P7 rat brain PFC. UMAP showing the integrated analysis of the single-nucleus transcriptomic and histone modalities of H3K4me1 (D) and H3K27me3 (E) Paired-Tag datasets, respectively. From left to right, transcriptome profiles, histone modification profiles, and integrated/combined snRNA-seq and histone modalities. Histone markers of the major cell types in H3K4me1 modification (F) and H3K27me3 modification (G).

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