Fig. 1: SARS-CoV-2 specific antibodies were detected in both vaccinated pregnant mice and their pups. | Communications Biology

Fig. 1: SARS-CoV-2 specific antibodies were detected in both vaccinated pregnant mice and their pups.

From: SARS-CoV-2 vaccination during pregnancy enhances offspring hippocampal neurogenesis and working memory via IFN-γ–responsive microglia

Fig. 1

a Diagram of the experimental procedure: pregnant mice were i.m. inoculated with SARS-CoV-2 vaccine with different dosages (50 μL and 100 μL) or PBS (100 μL) at embryonic day 14.5 (E14.5). The Spike RBD IgM antibody against SARS-CoV-2 was accurately measured by an ELISA kit in the serum of pregnant mice 3 days, 7 days and 14 days after vaccination, 50 μL for (b), 100 μL for (c). n = 8–11 mice/group. The RBD protein IgG inhibition ratio was assessed by an ELISA kit in the serum of pregnant mice 3 days and 14 days after SARS-CoV-2 vaccination, 50 (SCV-50) μL for (d), 100 (SCV-100) μL for (e). n = 10 mice/group. f An ELISA kit was used to quantify SARS-CoV-2 RBD-specific IgM antibodies in pup serum collected at postnatal day 28. Notably, IgM concentrations in pup samples were below the lower limit of detection (LOD) of the assay, n = 8 mice/group. g The RBD protein IgG inhibition ratio was assessed in peripheral serum at postnatal day 28, n = 8 mice/group. **p < 0.01, ***p < 0.001 calculated by two-way repeated measures ANOVA with Bonferroni’s tests (b, c, d, e), and one-way ANOVA followed by Tukey HSD test (g). Graphs indicate mean ± s.e.m.

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