Fig. 2: Presence of the H1-T6SS, but not T3SS, leads to increased c-di-GMP levels. | Communications Biology

Fig. 2: Presence of the H1-T6SS, but not T3SS, leads to increased c-di-GMP levels.

From: Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis

Fig. 2

A FRET measurement of c-di-GMP between a T3SS-deficient strain (ΔsctV) and WT. Data is shown as corrected FRET efficiency, a positively correlated indicator of c-di-GMP levels, see Suppl. Fig. 1. Detailed calculations see Methods. Each data point represents a measurement of a single cell. Experiments were repeated on multiple dates, with each color set representing data points from one day. Highest and lowest end of each bar represent maximum and minimum value, respectively; boxes indicate median and 25th–75th percentile. The total cells tested for each group is indicated under the respective bars. B FRET measurement of c-di-GMP between a H1-T6SS deletion strain (ΔH1-T6SS) and WT. Graph explanation is the same as above. Statistical analysis was done via Student’s t-test; ns, non-significant; ****, p < 0.0001.

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