Fig. 1: Schematic and validation of HybriSeq.
From: HybriSeq: probe-based device-free single-cell RNA profiling
a Multiple split probes are designed per transcript of interest. b Hybridization and ligation of split probes. c Labeling probes with unique cell barcodes via the split-and-pool method. Rounds 1 and 2 barcodes are ligated, and round 3 barcodes are added via PCR. d Sequencing results of 1:1 mixed HEK293 cells and Do-11-10 cells. 1.8% of barcodes contain probes targeting human and mouse transcripts. 98.2% of barcodes contain probes targeting only human or only mouse transcripts. e Scatter plot of average HybriSeq UMIs per cell in two independent biological replicates. Each dot represents a single probe. Pearson Correlation Coefficient (r = 0.992, p < 0.0001). f Scatter plot of gene-level average HybriSeq UMIs per cell and expression of the same gene obtained via bulk RNA-Seq. Each dot represents a gene-level measurement. Pearson Correlation Coefficient (r = 0.90, p < 0.0001).
