Fig. 2: Intra-transcript probing variation.

a–f Relative probe counts for probes targeting the specific transcript with standard deviation across all 394 cells are plotted for each probe. Gray lines are traces for individual cells, and the blue line is the average for each probe across all cells. The means of average counts for all probes are reported to the right with standard deviations. ^*For EIF2S2, only the first 11 out of 24 probes were considered in calculating the mean and standard deviation. For GHITM, only the first 17 out of 20 probes were considered in calculating the mean and standard deviation. g Average relative probe abundance normalized for each transcript for individual probes plotted against calculated probe specificity index (see “HybriSeq split probe design” in “Methods”). h Average relative probe abundance normalized for each transcript for individual probes plotted against CG content.