Fig. 3: Linear amplification with HybriSeq.
From: HybriSeq: probe-based device-free single-cell RNA profiling
a Left: model of UMIs/cell with n probes, detection efficiency e = 0.2, and N transcripts/cell from 1 to 8 transcripts/cell. Middle: model of the measurement standard deviation for UMIs/cell, right: model of the measurement SNR. The blue horizontal line denotes SNR = 2. b Left: experimentally obtained values for average NEFH UMIs/cell for the sampled number of probes n. Error bars represent the standard deviation associated with each measurement obtained by bootstrap via sampling the unique probes used to calculate average UMIs/cell. The green line represents the fitted linear curve with slope M. Middle: experimentally obtained values for the noise in expression measured with multiple probes n. The green line represents the fitted model from (Supplementary Fig. 7a) with the addition of a baseline variance to account for non-measurement-associated heterogeneity. Right: experimentally obtained values for the measurement SNR measured with multiple probes n. Error bars represent the standard deviation associated with each measurement obtained by bootstrap via sampling the unique probes used to calculate the average SNR. The green line represents the fitted model from the middle, and the blue horizontal line denotes SNR = 2. c Same as (b) for ARL5B. d Same as (b) for SCAF8.
