Fig. 4: Analyzing PBMC activation with HybriSeq.
From: HybriSeq: probe-based device-free single-cell RNA profiling
a UMAP of 11,148 stimulated and unstimulated PBMCs profiled with probes targeting 196 genes known to be important in classifying major cell types in PBMCs, as well as genes known to be differentially expressed in response to CD3/CD28-stimulated activation, colored by cell type. b Dot plot of genes used to determine cell type in (a). c UMAP same as (a), colored by the amount of time stimulated with CD3/CD28. Rest cells were not stimulated. d Violin plots of log-normalized expression for select genes that are differentially expressed at both 6 h and 24 h of CD3/CD28 stimulation compared to resting cells for B cells and CD4 T cells. Asterisks denote statistically significant (adjusted p-value < 0.01) differential expression between groups. n.s. denotes non-significant.
