Fig. 2: The Knockout of TRIM2 exacerbates II/R-induced intestinal apoptosis. | Communications Biology

Fig. 2: The Knockout of TRIM2 exacerbates II/R-induced intestinal apoptosis.

From: TRIM2 inhibits apoptosis by ubiquitinating BNIP3 to protect the intestine against ischemia-reperfusion injury in mice

Fig. 2

A Representative H&E-stained intestinal sections from WT mice and Trim2-/- mice under sham or II/R conditions (n = 6 per group). All images are presented at a scale of 100 μm. B Representative images for TUNEL staining of the intestine in the four groups of mice (n = 6 per group), with a scale bar of 100 μm. C Representative photomicrographs of immunohistochemical staining of the intestine in four groups of mice using antibodies against TRIM2, Bax, Bcl2, Bad, and cleaved Casp3 (n = 6 per group). The scale bars are 200 μm. D The protein expressions of TRIM2, Bax, Bcl2, BAD, and cleaved Casp3 in the four groups of mice were detected by Western blot in the intestine. β-actin was served as a loading control. (E) The morphological changes of mitochondria in the intestinal tissues of the four groups of mice were observed by TEM. Scale bars: 25 µm (overview), 5 µm (mid-magnification), 2.5 µm (high-magnification).

Back to article page