Fig. 3: Reducing TRIM2 expression promotes H/R-induced apoptosis in intestinal epithelial cells. | Communications Biology

Fig. 3: Reducing TRIM2 expression promotes H/R-induced apoptosis in intestinal epithelial cells.

From: TRIM2 inhibits apoptosis by ubiquitinating BNIP3 to protect the intestine against ischemia-reperfusion injury in mice

Fig. 3

A Lactate dehydrogenase (LDH) activity in the supernatants of IEC-6 and Caco-2 cells following H/R treatment (n = 4 biologically independent samples). B The protein expression levels of TRIM2, Bax, Bcl2, Bad and cleaved Casp3 in IEC-6 and Caco-2 TRIM2-knockdown cells were determined by Western blotting following H/R treatment. β-actin was used as a loading control. C Quantification of the TRIM2, Bax, Bcl2, Bad and cleaved Casp3 western blot bands (n = 6 biologically independent samples). D BAD mRNA levels in IEC-6 and Caco-2 TRIM2-knockdown cells subjected to control or H/R treatment (n = 3 biologically independent samples). E Flow cytometry was employed to ascertain the extent of apoptosis in IEC-6 (n = 8 biologically independent samples) and Caco-2 (n = 6 biologically independent samples) TRIM2-knockdown cells subjected to control or H/R treatment. All results are expressed as the mean ± SD. Statistical significance was determined using one-way ANOVA followed by Tukey’s test, with the following levels of significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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