Fig. 4: Overexpression of TRIM2 restrains H/R-induced apoptosis in intestinal epithelial cells. | Communications Biology

Fig. 4: Overexpression of TRIM2 restrains H/R-induced apoptosis in intestinal epithelial cells.

From: TRIM2 inhibits apoptosis by ubiquitinating BNIP3 to protect the intestine against ischemia-reperfusion injury in mice

Fig. 4

A LDH activity in IEC-6 and Caco-2 cell supernatants following H/R treatment (n = 4 biologically independent samples). B Following transfection with either the HA-Vector or the HA-TRIM2 plasmid, IEC-6 and Caco-2 cells were stained with JC-1 for a period of 20 minutes at 37 °C. The cells were then observed using a laser scanning confocal microscope. Scale bars are 100 μm (IEC-6) and 50 μm (Caco-2). C The protein expression levels of TRIM2, BAX, Bcl2, BAD and cleaved Casp3 in IEC-6 and Caco-2 TRIM2-overexpression cells were determined by Western blot following H/R treatment. β-actin was used as a loading control. D Flow cytometry was employed to ascertain the extent of apoptosis in IEC-6 and Caco-2 TRIM2-overexpression cells subjected to control or H/R treatment (n = 6 biologically independent samples). All results are expressed as the mean ± SD. Statistical significance was determined using one-way ANOVA followed by Tukey’s test, with the following levels of significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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