Fig. 5: Transcription factor regulatory network of hepatic progenitor-like cells.

A Transcription factor motifs enriched in SH5 calculated by marker peaks. B The expression of enriched transcription factors in PHx scRNA-seq with only hepatocytes. Color indicates relative expression across all time points and dot size indicates the percentage of cells in that time point expressing the gene. P was calculated by Wilcoxon. C Prioritization of gene targets for Nfe2l2. The x axis shows Pearson correlation between TF motif activity and integrated gene expression for all expressed genes across all hepatocytes; the y axis shows TF linkage score (for all linked peaks, sum of motif score scaled by peak-to-gene link correlation). Color of points indicates hypergeometric enrichment of the TF motif in all linked peaks for each gene. D The expression of marked genes in (C) in PHx scRNA-seq with only hepatocytes. Color indicates relative expression across all time points and dot size indicates the percentage of cells in that time point expressing the gene. P was calculated by Wilcoxon. E GO term enrichments for positive target genes of Nfe2l2. F The lollipop plots representing the positive transcription factors, whose TF motif activity and matched gene expression are positively correlated across hepatocyte subtypes. The dot color indicates the P. G Genes shared by proliferating cells and progenitor-like cells in the PHx model with LPLCs in the DDC model showing in the Venn plot. H Regulatory networks of the AP-1 family and its target genes. I GO term enrichments for the target genes of AP-1 family.