Fig. 3: Disruption of mitochondrial OXPHOS leads to activation of intestinal PKA in C. elegans. | Communications Biology

Fig. 3: Disruption of mitochondrial OXPHOS leads to activation of intestinal PKA in C. elegans.

From: Genome-wide RNAi screening in C. elegans reveals OXPHOS and pyrimidine synthesis pathways as PKA regulators

Fig. 3

A The schematic illustrating mitochondrial OXPHOS, including the loci of NUO-1, CYC-1, and R53.4, as well as the target of rotenone. B Representative images (left) and quantitative results (right) of measurements of the intestinal PKA activity in response to RNAi against mitochondrial OXPHOS genes. The scale bar represents 100 μm. C Western blot analysis of nuo-1, cyc-1 and R53.4 RNAi worms. D Representative images (left) and quantitative results (right) for the PKA sensor in response to rotenone treatment. Worms were treated with 4 μM Rotenone from the L4 stage for 12 h. The scale bar is 100 μm. E Western blot analysis of worms treated with rotenone.For assays using the PKA sensor, N = 3 independent experiments containing at least 30 worms per condition. The statistical significance values were determined by t-test and one-way ANOVA analysis, followed by Dunnett’s multiple comparisons test. Error bars denote the SEM.

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