Fig. 5: Distribution of periaxin, ezrin, and F-actin in primary lens culture cells in vitro.
From: Periaxin gene variants are linked to age-related cataracts in Cx46 deficient lenses
a Fluorescent confocal images show that 129-PRX proteins (red) colocalize with ezrin (green) and F-actin (purple), forming a distinct stripe-like network at the surface of differentiated fiber-like cells in vitro. These cells were derived from primary cultured lens epithelial cells of 129-WT mice and treated with 200 ng/ml bFGF in culture medium for 2 weeks. In the Z-axis image, the horizontal surface plane is indicated by an arrowhead. Scale bar: 10 μm. b Colocalization of 129-Prx-mEOS protein with F-actin in primary cultured lens epithelial cells transfected with 129-Prx-mEOS, co-labeled with rhodamine-phalloidin for F-actin. Primary cells were isolated from 129-WT mouse lenses. Scale bar: 10 μm.
