Fig. 4: Results of selective sweep analysis and functional validation of the Gld gene.
From: Demography and adaptation of a species specific pollinator associated with an invasive fig tree
Results of selective sweep analyses (a–e) and in vitro functional validation of the glucose dehydrogenase (Gld) (f). a–d Manhattan plots showing the distribution of XP-CLR, FST, π ratio and Tajima’s D values in each contig. The non-overlapping windows with top 5% values for XP-CLR, FST and π ratio (above the red dashed lines) and negative values for Tajima’s D were considered (below the red dashed line) as regions under positive selection. The genes that are supported by all the four statistics and relevant with adaptation to the nonnative range are marked (Supplementary Table 6). e Venn diagram of positively selected genes revealed by the four statistics (Supplementary Table 6). f Standard of D-glucono-1,5-lactone (at the concentration of 100 ng/ml) and reaction products (treatments with enzyme added for three replicates) are identified using UPLC (see Methods).
