Fig. 3: Spatial postural deflection of RIM-BP2 during action potentials in cortical neurons and SH-SY5Y cells.
From: Visualization of RIM-BP2’s crane-like function in neuronal vesicle transport using FRET
A Fluorescence images of BKTS, BTS, RKTS, and RTS biosensors expressed in rat cortical neurons. YPet/ECFP FRET ratio images before and after electrical stimulation are shown. B Trends of normalized FRET ratio for BKTS, BTS, RKTS and RTS biosensors in cortical neurons. Red arrows indicate stimulus onset. BKTS, n = 7; BTS, n = 5; RKTS, n = 8; RTS, n = 10. C Comparative analysis of FRET ratio values at 600 s post-stimulation, normalized to pre-stimulation baseline. D, E YPet/ECFP FRET ratio images of BKTS, BTS (D) and RKTS, RTS (E) in SH-SY5Y cells under high K+ solution stimulation and electrical stimulation. F Trend of YPet/ECFP FRET ratio for BKTS, BTS, RKTS and RTS under high K+ solution stimulation (BKTS, n = 8; BTS, n = 6; RKTS, n = 8; RTS, n = 6) and electrical stimulation (BKTS, n = 8; BTS, n = 5; RKTS, n = 12; RTS, n = 7). G FRET ratio changes of biosensors in SH-SY5Y cells during electrical stimulation. Red circles: baseline FRET ratio before stimulation; Blue circles: FRET ratio at 300 s. Black lines connect measurements of the same cell. H Comparative analysis of BKTS and RKTS biosensors in SH-SY5Y cells corresponding to two kinds of stimulations. Student’s t test, ***P < 0.001, ns P > 0.05. Data are mean ± SEM.
