Fig. 1: Development of a reproducible system for the induction of senescence phenotype and measuring compound potency using fluorescence detection. | Communications Biology

Fig. 1: Development of a reproducible system for the induction of senescence phenotype and measuring compound potency using fluorescence detection.

From: An effective system for senescence modulating drug development using quantitative high-content analysis and high-throughput screening

Fig. 1

a Representative bright-field images of WI38 cells with SA-β-gal staining after MMC-induced senescence. b Fluorescent confocal images of X-gal precipitation with EX640/EM665-705 nm. c Analysis of SA-β-gal positive cells in bright-field images manually and in fluorescence images automatically. d Representative images of SA-β-gal activity in senescent WI38 cells treated with rapamycin at different concentrations. e Dose-response curve of rapamycin on cellular senescence. f IC50 values for the inhibitory effect of rapamycin on SA-β-gal activity from three independent experiments. All scale bars are 100 µm. P values were determined by Unpaired t-test. Data are presented as mean ± SD. ***P < 0.001.

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