Fig. 2: Compound screening using the automated fluorescence high throughput screening workflow.

a Flowchart of the high-throughput screening experiment workflow for senescence modulators. b Representative fluorescent images of SA-β-gal signals after treatment by senomorphic, senolytic and seno-inducer candidates. Control is without MMC treatment while all others have been treated with MMC. c Scatter plot showing the SenoScore of 2520 compounds, where the yellow and green data points are positive seno-inducers and inhibitors selected from PositiveRate method for comparison, respectively.