Fig. 1: Expression profile of Stip1 and associated genes during early mouse development evidenced by single-cell RNA sequencing. | Communications Biology

Fig. 1: Expression profile of Stip1 and associated genes during early mouse development evidenced by single-cell RNA sequencing.

From: Stress-inducible phosphoprotein 1 (STIP1) is a critical stemness regulator in mouse embryonic stem cells and early mammalian development

Fig. 1

A Cells from the zygote to the late blastocyst projected onto a principal component analysis (PCA) plot and colored according to their developmental stage. B Violin plots with boxplot display Stip1 expression (normalized to zygote levels) across pre-implantation stages. Wilcoxon test, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 for specific stages relative to all others. Boxplots depict the quartiles and extend from the minimum and maximum data points within 1.5 times the interquartile range. C PCA shown in (A), colored to indicate the scaled expression of Stip1, Sox2, Pou5f1/Oct4, Nanog and Klf4. D Pearson correlation plot highlighting genes that are positively (red) or negatively (blue) correlated with Stip1, while non-significant correlations are shown in light gray. E Gene Ontology overrepresentation analysis of genes positively (red) and negatively (blue) correlated with Stip1. F Pearson’s correlation coefficient between Stip1 and key pluripotency factors: Pou5f1 (R = 0.41), Sox2 (R = 0.23) and Nanog (R = 0.47).

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