Fig. 2: STIP1 modulation alters the transcriptional profile of mESCs.

A Schematic representation showing wild-type (WT), STIP1^+/− (50% expression) and STIP1^TgA (overexpression) mESCs lines derived from the inner cell mass of the blastocyst of genetically modified Stip1 mice. Total RNA extracted from these mESCs was subjected to bulk RNA sequencing followed by functional profiling. B Volcano plot of differentially expressed genes (DEGs) in STIP1^TgA versus (vs) WT. DEGs are classified as non-significant (gray), up- (red) or downregulated (blue), with genes of interest highlighted, p < 0.05. C Overrepresentation analysis (ORA) of Gene Ontology (GO) terms enriched among the DEGs of STIP1^TgA mESCs. D Volcano plot of non-significant (gray), up- (red) or downregulated (blue) DEGs of STIP1^+/− vs WT, p < 0.05. E ORA of GO terms enriched among the DEGs of STIP1^+/−, sorted by p-value. F Volcano plot of non-significant (gray), up- (red) and downregulated (blue) DEGs of STIP1^TgA vs STIP1^+/−, p < 0.05. G ORA of GO terms enriched among the DEGs of STIP1^TgA relative to STIP1^+/−, sorted by p-value.