Fig. 4: Metabolomics analysis of oxyR in P. aeruginosa. | Communications Biology

Fig. 4: Metabolomics analysis of oxyR in P. aeruginosa.

From: Systems-level exploitation of OxyR regulon unravels a potential antibacterial target in Pseudomonas aeruginosa

Fig. 4

A Score plot of OPLS-DA (orthogonal projections to latent structures-discriminant analysis) model obtained from PAO1 and oxyR metabolic profiling data (R2X = 0.491, R2Y = 0.885, Q2 = 0.549). Both groups showed significant separation and most data sets are located in Hotelling’s T-squared ellipse ( > 95%), with one exception in PAO1 data set. (B) Pathway enrichment analysis of oxyR metabolites as compared to PAO1. C Differential metabolites in oxyR mutant. Gray bars, solvents; blue bars, amino acids; Khaki bars, amino acid derivatives; orange bars, nucleotides; pink bars, groups of reduced metabolites. Data are means ± SEM (n = 6). D Spot assay of amino acids in growth complement of oxyR mutant under aerobic condition. 5 μM methionine (Met), cysteine (Cys), and aspartate (Asp) were used in a final concentration supplemented in LB agar plates (CK). The overnight bacterial cultures were diluted from 109 to 102 per ml.

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